The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism inPseudomonas putida

Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440

The development of efficient recovery processes is essential to reduce the cost of polyhydroxyalkanoates (PHAs) production. In this work, a programmed self-disruptive Pseudomonas putida BXHL strain, derived from the prototype medium-chain-length PHA producer bacterium P. putida KT2440, was constructed as a proof of concept for exploring the possibility to control and facilitate the release of P...

An alternative sigma factor governs the principal sigma factor in Streptomyces griseus.

In bacteria, the RNA polymerase holoenzyme comprises a five-subunit core enzyme and a dissociable subunit, sigma factor, which is responsible for transcriptional initiation. The filamentous bacterium Streptomyces griseus has 52 sigma factors, including one essential 'principal' sigma factor (σ(HrdB) ) that is responsible for the transcription of housekeeping genes. Here we characterized an alte...

Knock - out of extracytoplasmic function sigma factor ECF - 10 affects stress resistance 1 and biofilm formation in Pseudomonas putida

Functional metagenomics using Pseudomonas putida expands the known diversity of polyhydroxyalkanoate synthases and enables the production of novel polyhydroxyalkanoate copolymers

Functional metagenomics using Pseudomonas putida expands the known diversity of 1 polyhydroxyalkanoate synthases and enables the production of novel polyhydroxyalkanoate 2 copolymers 3 4 14 15. CC-BY 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not. Abstract 17 Bacterially produced biodegradable pol...

The alternative sigma factor sigma B and the virulence gene regulator PrfA both regulate transcription of Listeria monocytogenes internalins.

Some Listeria monocytogenes internalins are recognized as contributing to invasion of mammalian tissue culture cells. While PrfA is well established as a positive regulator of L. monocytogenes virulence gene expression, the stress-responsive sigma(B) has been recognized only recently as contributing to expression of virulence genes, including some that encode internalins. To measure the relativ...